Journal: Cancer

Article Title: A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer.

doi: 10.1002/cncr.23057

Figure Lengend Snippet: FIGURE 1. Reactivity of superparamagnetic particles coated with p53 (amino

Article Snippet: Superparamagnetic particles with carboxylic acid groups (Dynabeads M-270; Invitrogen, Krefeld, Germany) were coated with p53 peptides from the NH2 terminus (peptide 1; amino acid [AA]15-AA29), the COOH terminus (peptide 2; AA314-AA326), the wildtype p53 protein (Dianova), and a monoclonal antibody to p53 (mouse antihuman p53 clone DO-2; Serotec, Dusseldorf, Germany).

Techniques:

Journal: Cancer

Article Title: A highly sensitive particle agglutination assay for the detection of P53 autoantibodies in patients with lung cancer.

doi: 10.1002/cncr.23057

Figure Lengend Snippet: FIGURE 2. Detection of p53 autoantibodies (Abs) in randomly selected

Article Snippet: Superparamagnetic particles with carboxylic acid groups (Dynabeads M-270; Invitrogen, Krefeld, Germany) were coated with p53 peptides from the NH2 terminus (peptide 1; amino acid [AA]15-AA29), the COOH terminus (peptide 2; AA314-AA326), the wildtype p53 protein (Dianova), and a monoclonal antibody to p53 (mouse antihuman p53 clone DO-2; Serotec, Dusseldorf, Germany).

Techniques:

Journal: Journal of Radiation Research

Article Title: Carbon-ion irradiation overcomes HPV-integration/E2 gene-disruption induced radioresistance of cervical keratinocytes

doi: 10.1093/jrr/rrz048

Figure Lengend Snippet: Expression of p53 after [ A ] photon-RT and [ B ] 12 C-RT. Expression of p53 was analysed 24 h after irradiation with either photons or 12 C. C33a: 2/7 Gy photons → 1.4-fold ( P = 0.0035)/1.3-fold ( P = 0.0037) increase. 0.5/2 Gy 12 C → 1.1-fold ( P = 0.0066)/1.2-fold ( P = 0.0001) increase. Caski: 2/7 Gy photons → 2.3-fold ( P = 0.0097)/2.8-fold ( P = 0.003) increase. 0.5/1/2 Gy 12 C → 3.4-fold ( P = 0.0508)/5.4-fold (ns)/4.2-fold ( P = 0.0259) increase. W12: 2/7 Gy photons → 0.5-/0.3-fold decrease (ns). 0.5/1/2 Gy 12 C → 0.8-/0.9-/0.9-fold decrease (ns). S12: 2/7 Gy photons → 1.9-fold ( P = 0.00722)/3.4-fold ( P = 0.0526) increase. 0.5/1/2 Gy 12 C → 1.6-fold ( P = 0.0178)/1.3-fold ( P = 0.0043)/1.7-fold ( P = 0.0292) increase. ns = not significant.

Article Snippet: The same procedure was performed for p53 staining using 20 μl of Fluorochrome-conjugated antibody p53-ak (FITC mouse anti-human p53 antibody from Becton Dickinson, Franklin Lakes, NJ, USA)

Techniques: Expressing, Irradiation

Journal:

Article Title: The Use of Laser Scanning Cytometry to Assess Depth of Penetration of Adenovirus p53 Gene Therapy in Human Xenograft Biopsies

doi:

Figure Lengend Snippet: Effect of paclitaxel on Ad5/p53 induction of apoptosis. Five tumors, one per mouse, for each treatment group were scanned using LSC. For each group, 50,000 total nuclei were scanned. Group mean percentages of apoptotic nuclei are shown; error bars represent the maximal percent coefficient of variance of the weighted means.

Article Snippet: The primary antibodies used were mouse anti-human p53 (NovoCastra) and mouse anti-human p21 (PharMingen, San Diego, CA).

Techniques:

Journal: Molecular Biology of the Cell

Article Title: Lens Fiber Cell Differentiation and Denucleation Are Disrupted through Expression of the N-Terminal Nuclear Receptor Box of Ncoa6 and Result in p53-dependent and p53-independent Apoptosis

doi: 10.1091/mbc.E09-12-1031

Figure Lengend Snippet: Abnormal lens fiber cell apoptosis is both p53-dependent and p53-independent. (A) Western blot was performed to analyze p53 protein expression in the lens and eyeball (without lens) of WT and transgenic mice at neonatal stage. β-Actin was used for loading control. (B) External observation of adult mouse eyes (a–f) and histological analysis of E16.5 mouse eyes (g–i) in p53 +/+ ; Cryaa-dnNCOA6 , p53 + /null ; Cryaa-dnNCOA6 , p53 null/null ; Cryaa-dnNCOA6 , and WT mice. (C) Reduced number of DSBs in p53 null/null ; Cryaa-dnNCOA6 lenses (d–f) compared with p53 +/+ ; Cryaa-dnNCOA6 lenses at E14.5 (a–c). Nuclei (blue signal) were stained with DAPI. Scale bar is shown in a and d. (D) To quantify the amounts of DSBs in Cryaa-dnNCOA6 lenses in WT or p53 null background, the number of γ-H2AX-positive nuclei was compared between E14.5 p53 +/+ ; Cryaa-dnNCOA6 (n = 10) and p53 null/null ; Cryaa-dnNCOA6 (n = 9) lenses. Nonspecific signals outside of the lens are explained in . Lens epithelium, LE; transitional zone, TZ.

Article Snippet: The dilution used for each antibody is listed as follows: mouse monoclonal anti-FLAG antibody (mAb) (1:1000; Sigma-Aldrich), rabbit polyclonal anti-c-Maf antibody (1:1000; Bethyl Laboratories, Montgomery, TX), mouse monoclonal anti-p53 antibody (1:250; Merck, Whitehouse Station, NJ), rabbit polyclonal anti-αA-crystallin antibody (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-αB-crystallin antibody (1:2000; Assay Designs, Ann Arbor, MI), rabbit polyclonal anti-β-crystallin antibody (1:500; Santa Cruz Biotechnology), rabbit polyclonal anti-γ-crystallin antibody (1:500; Santa Cruz Biotechnology), monoclonal anti-β-actin antibody (1:2000; Sigma-Aldrich), and mouse monoclonal anti-NCOA6 antibody (1:1000; produced in Dr. Jianming Xu's laboratory).

Techniques: Western Blot, Expressing, Transgenic Assay, Staining

Journal: Molecular Biology of the Cell

Article Title: Lens Fiber Cell Differentiation and Denucleation Are Disrupted through Expression of the N-Terminal Nuclear Receptor Box of Ncoa6 and Result in p53-dependent and p53-independent Apoptosis

doi: 10.1091/mbc.E09-12-1031

Figure Lengend Snippet: Summary diagram of lens fiber cell differentiation and the role of NCOA6 in the denucleation process. In WT lens (left side of the diagram), up-regulation of c-Maf and crystallins is essential for lens fiber cell differentiation. Their terminal differentiation requires orchestrated degradation of all subcellular organelles to avoid light scattering. Nuclear degradation is a relatively lengthy process (3–4 d in mouse; ). In Cryaa-dnNCOA6 transgenic lenses (right side of the diagram), p53-dependent and p53-independent proapoptotic processes become evident as early as at E13.5 due to the generation of CCAP3 followed by the appearance of DSBs monitored via γ-H2AX–specific antibodies. Although abundant TUNEL signals are found at E16.5, the nuclear degradation is not properly executed and abnormally differentiated lens fibers, with significantly reduced expression of c-Maf and γ-crystallins and retained nuclei, are preserved in the transgenic lenses.

Article Snippet: The dilution used for each antibody is listed as follows: mouse monoclonal anti-FLAG antibody (mAb) (1:1000; Sigma-Aldrich), rabbit polyclonal anti-c-Maf antibody (1:1000; Bethyl Laboratories, Montgomery, TX), mouse monoclonal anti-p53 antibody (1:250; Merck, Whitehouse Station, NJ), rabbit polyclonal anti-αA-crystallin antibody (1:2000; Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-αB-crystallin antibody (1:2000; Assay Designs, Ann Arbor, MI), rabbit polyclonal anti-β-crystallin antibody (1:500; Santa Cruz Biotechnology), rabbit polyclonal anti-γ-crystallin antibody (1:500; Santa Cruz Biotechnology), monoclonal anti-β-actin antibody (1:2000; Sigma-Aldrich), and mouse monoclonal anti-NCOA6 antibody (1:1000; produced in Dr. Jianming Xu's laboratory).

Techniques: Cell Differentiation, Transgenic Assay, TUNEL Assay, Expressing

Journal: Oncotarget

Article Title: Methylation of CpG sites in the upstream regulatory region, physical status and mRNA expression of HPV-6 in adult-onset laryngeal papilloma

doi: 10.18632/oncotarget.19898

Figure Lengend Snippet: Clinicopathological characteristics of 11 patients with AO-LP

Article Snippet: The sections were subsequently incubated overnight at 4 °C with primary monoclonal mouse anti-p16 INK4a antibody (MTM Laboratories AG, Heidelberg, Germany) for p16 INK4a staining, primary monoclonal mouse anti-p53 antibody (1:500; Progen Biotech GmbH, Heidelberg, Germany) for p53 staining, and primary monoclonal mouse anti-pRb antibody (1:2000; Life-Span BioSciences, Seattle, WA) for pRb staining.

Techniques:

Journal: Oncotarget

Article Title: Methylation of CpG sites in the upstream regulatory region, physical status and mRNA expression of HPV-6 in adult-onset laryngeal papilloma

doi: 10.18632/oncotarget.19898

Figure Lengend Snippet: Positive expression was defined as p16INK4a staining in > 40%, or p53 and pRb staining in > 25% of 2000 tumor cells. The six micrographs show the typical p16, p53, and pRb immunoreactivity patterns corresponding to positive and negative expression (×100; bar, 100 μm).

Article Snippet: The sections were subsequently incubated overnight at 4 °C with primary monoclonal mouse anti-p16 INK4a antibody (MTM Laboratories AG, Heidelberg, Germany) for p16 INK4a staining, primary monoclonal mouse anti-p53 antibody (1:500; Progen Biotech GmbH, Heidelberg, Germany) for p53 staining, and primary monoclonal mouse anti-pRb antibody (1:2000; Life-Span BioSciences, Seattle, WA) for pRb staining.

Techniques: Expressing, Staining